Hook effect in immunoradiometric assay of CA 125 in peritoneal fluid: evidence of high CA 125 concentrations by a comparative immunoblotting analysis

"High-dose hook effect" with the Centocor CA 125 assay.

Quantification of CA 125 in peritoneal fluid: inhibition of the CA 125 response in the one-step immunoradiometric assay and establishment of a two-step assay format.

Serum and Peritoneal Fluid CA 125 Levels Infertile Women with Pelvic Endometriosis in Urummia

SUMMARY Infertility has a special importance in gynecology in causing social, psychological and somatic prohlems. Endometriosis is a benign but progressive disorder in reproductive life and is more common in infertile women. CA 125 is a cell membrane glycoprotein. The endometrium is one the known source of this antigen and seems to be elevated in the pelvic endometriosis. In this study, from...

CA 125 secretion by peritoneal mesothelial cells.

AIMS To investigate the secretion of the tumour marker CA 125 by cultured human mesothelial cells; to determine if secretion of CA 125 could be observed by activating the mesothelial monolayers with different cytokines. METHODS Mesothelial cells were isolated from human omentum and cultured to confluent monolayers on perforated polycarbonate membranes (pore size 0.4 micron). The mesothelial m...

Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 125) associated with human epithelial ovarian carcinomas.

CA 125 is an antigenic determinant expressed by greater than 80% of nonmucinous epithelial ovarian carcinomas. An immunoradiometric assay has been developed using a murine monoclonal antibody (OC125) to quantitate CA 125 in human serum. This immunoradiometric assay was optimized for specificity, sensitivity, and performance characteristics. Using a simultaneous immunoradiometric assay, the mean...